Hotspot

atpF-atpH

LSC · 15732-16332 · 601 bp

Back to Prosopis

Pi 0.0594

MarkerSeek score 91.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 601
variable_sites: 34
pi: 0.0594
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 91.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TGGGCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	717-719 bp	1.0	82.7
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	AGGCAGAGGGAAAAATACGG	50.0	50.0	57.9	57.6	784-786 bp	1.0	82.1
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGGGG	50.0	55.0	57.9	58.6	782-784 bp	1.0	80.6
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GGCAGAGGGAAAAATACGGG	50.0	55.0	57.9	58.6	783-785 bp	1.0	80.6
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CCGAGGCAGAGGGAAAAATA	50.0	50.0	57.9	57.3	787-789 bp	1.0	79.8