Hotspot

trnS-GCU-trnR-UCU

LSC · 9995-12314 · 2320 bp

Back to Prosopis

Pi 0.0478

MarkerSeek score 80.90

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 2320
variable_sites: 106
pi: 0.0478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	2317-2391 bp	1.0	70.7
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	2316-2390 bp	1.0	70.7
trnS-GCU-trnR-UCU_p3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	2323-2397 bp	1.0	70.0
trnS-GCU-trnR-UCU_p4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	2322-2396 bp	1.0	70.0
trnS-GCU-trnR-UCU_p5	ACGGAAAGAGAGGGATTCGA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.2	56.9	2371-2445 bp	1.0	69.4