Hotspot

trnH-GUG-psbA

LSC · 701-1092 · 392 bp

Back to Prosopis

Pi 0.11

MarkerSeek score 79.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 41
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GGTATCTGGTTTACCGCGTT	55.0	50.0	58.1	58.0	633-634 bp	1.0	86.6
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GGTATCTGGTTTACCGCGTT	50.0	50.0	57.9	58.0	647-648 bp	1.0	86.4
trnH-GUG-psbA_p3	ACTTCTCGCTACCTATGGCT	GGTATCTGGTTTACCGCGTT	50.0	50.0	57.9	58.0	951 bp	0.5	59.3
trnH-GUG-psbA_p4	TCACGGTACCTATGATGGCT	GGTATCTGGTTTACCGCGTT	50.0	50.0	57.9	58.0	1163 bp	0.5	59.2
trnH-GUG-psbA_p5	TATGATGGCTGTGACTGGGA	GGTATCTGGTTTACCGCGTT	50.0	50.0	58.1	58.0	1153 bp	0.5	58.9