Hotspot

clpP

LSC · 70268-72324 · 2057 bp

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Pi 0.0177

MarkerSeek score 56.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2057
variable_sites: 53
pi: 0.0177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	AAACGGCCAGGATCATTCAA	50.0	45.0	58.0	57.8	2861-2881 bp	1.0	80.9
clpP_p2	CTGAACGAGTCGCACATACA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	2825-2845 bp	1.0	80.7
clpP_p3	TGATTGGCTCTCGTGTGTTT	ATACTCCAACCACCCCATGA	45.0	50.0	57.7	58.0	2961-2981 bp	1.0	80.5
clpP_p4	TTGATTGGCTCTCGTGTGTT	ATACTCCAACCACCCCATGA	45.0	50.0	57.7	58.0	2962-2982 bp	1.0	80.5
clpP_p5	TTTGATTGGCTCTCGTGTGT	ATACTCCAACCACCCCATGA	45.0	50.0	57.7	58.0	2963-2983 bp	1.0	80.5