Hotspot

ndhF-rpl32

SSC · 112397-113490 · 1094 bp

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Pi 0.0232
MarkerSeek score 65.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1094
variable_sites
36
pi
0.0232
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCGGTGGGAAAAAGTA TTTTGAGATAGAAGTACGTTTTT 45.0 26.1 57.9 52.4 1016-1229 bp 1.0 46.5
ndhF-rpl32_p2 TGTTGCGGTGGGAAAAAGTA TTTTTGAGATAGAAGTACGTTTT 45.0 26.1 57.9 52.4 1017-1230 bp 1.0 46.5
ndhF-rpl32_p3 TGTTGCGGTGGGAAAAAGTA TTTTTGAGATAGAAGTACGTTTTT 45.0 25.0 57.9 53.2 1017-1230 bp 1.0 46.5
ndhF-rpl32_p4 ATCTTTTGTTGCGGTGGGAA TTTTGAGATAGAAGTACGTTTTT 45.0 26.1 58.3 52.4 1022-1235 bp 1.0 46.5
ndhF-rpl32_p5 ATCTTTTGTTGCGGTGGGAA TTTTTGAGATAGAAGTACGTTTT 45.0 26.1 58.3 52.4 1023-1236 bp 1.0 46.5