Hotspot

petA-psbJ

LSC · 63327-64161 · 835 bp

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Pi 0.0216

MarkerSeek score 71.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 26
pi: 0.0216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATTACGCGTACAAGGCCTTT	GCCGATACTACTGGAAGGAT	45.0	50.0	57.9	56.2	1043-1075 bp	1.0	68.9
petA-psbJ_p2	TTACGCGTACAAGGCCTTTT	GCCGATACTACTGGAAGGAT	45.0	50.0	58.1	56.2	1042-1074 bp	1.0	68.8
petA-psbJ_p3	ATTACGCGTACAAGGCCTTT	GCCGATACTACTGGAAGGATT	45.0	47.6	57.9	57.0	1043-1075 bp	1.0	67.0
petA-psbJ_p4	TTACGCGTACAAGGCCTTTT	GCCGATACTACTGGAAGGATT	45.0	47.6	58.1	57.0	1042-1074 bp	1.0	66.9
petA-psbJ_p5	ACAAGGCCTTTTGCTCTTCT	GCCGATACTACTGGAAGGAT	45.0	50.0	57.6	56.2	1034-1066 bp	1.0	66.8