Pi
0.02
MarkerSeek score
67.80
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GACCACCGCATTACAAATGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 690-721 bp | 1.0 | 85.4 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 672-703 bp | 1.0 | 85.1 |
| trnT-UGU-trnL-UAA_p3 | GACCACCGCATTACAAATGC | CGCTCGGTAGGTTTCCATAC | 50.0 | 55.0 | 58.1 | 58.2 | 745-776 bp | 1.0 | 84.0 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | CGCTCGGTAGGTTTCCATAC | 55.0 | 55.0 | 57.9 | 58.2 | 727-758 bp | 1.0 | 83.8 |
| trnT-UGU-trnL-UAA_p5 | AAAAGCCAGCTATCGGAGTC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.7 | 58.0 | 718-749 bp | 1.0 | 83.5 |