Hotspot

ycf3-trnS-GGA

LSC · 44509-45220 · 712 bp

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Pi 0.014
MarkerSeek score 70.10
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
712
variable_sites
14
pi
0.014
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CTCTTTCTCGCCCGAAGTTG AGGTGAAGGGTACGGAAAGA 55.0 50.0 58.9 58.0 786-913 bp 1.0 77.7
ycf3-trnS-GGA_p2 CTCTTTCTCGCCCGAAGTTG CCCTCGGTAAACAAAAGCCT 55.0 50.0 58.9 58.1 754-881 bp 1.0 77.2
ycf3-trnS-GGA_p3 CTCTTTCTCGCCCGAAGTTG ACGGAAAGAGAGGGATTCGA 55.0 50.0 58.9 58.2 775-902 bp 1.0 76.7
ycf3-trnS-GGA_p4 CTTTCTCGCCCGAAGTTGT AGGTGAAGGGTACGGAAAGA 52.6 50.0 58.1 58.0 784-911 bp 1.0 76.4
ycf3-trnS-GGA_p5 TTTCTCGCCCGAAGTTGTC AGGTGAAGGGTACGGAAAGA 52.6 50.0 58.1 58.0 783-910 bp 1.0 76.4