Hotspot

trnS-UGA-psbZ

LSC · 35342-35615 · 274 bp

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Pi 0.00243

MarkerSeek score 34.10

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 274
variable_sites: 1
pi: 0.00243
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	CACCCATCAGGAGAAGCAAA	55.0	50.0	58.1	57.8	408-425 bp	1.0	78.1
trnS-UGA-psbZ_p2	CTATCAACCACTCGGCCATC	CACCCATCAGGAGAAGCAAA	55.0	50.0	58.1	57.8	397-414 bp	1.0	77.5
trnS-UGA-psbZ_p3	AAGACCGGAGCTATCAACCA	CACCCATCAGGAGAAGCAAA	50.0	50.0	58.4	57.8	407-424 bp	1.0	75.1
trnS-UGA-psbZ_p4	GGTTTTCAAGACCGGAGCTA	CACCCATCAGGAGAAGCAAA	50.0	50.0	57.5	57.8	414-431 bp	1.0	74.8
trnS-UGA-psbZ_p5	TATCAACCACTCGGCCATCT	CACCCATCAGGAGAAGCAAA	50.0	50.0	58.5	57.8	396-413 bp	1.0	74.5