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psbC-trnS-UGA

LSC · 35009-35248 · 240 bp

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Pi 0.0847

MarkerSeek score 92.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 30
pi: 0.0847
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 92.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCATTTATGGCACGCTGGAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.9	58.1	420 bp	1.0	88.3
psbC-trnS-UGA_p2	TCATTTATGGCACGCTGGAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.9	58.2	366 bp	1.0	87.9
psbC-trnS-UGA_p3	TCATTTATGGCACGCTGGAA	GATGGCCGAGTGGTTGATAG	45.0	55.0	57.9	58.1	431 bp	1.0	87.5
psbC-trnS-UGA_p4	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	390 bp	1.0	86.7
psbC-trnS-UGA_p5	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	389 bp	1.0	86.1