Hotspot

trnS-GCU-trnG-UCC

LSC · 8001-8672 · 672 bp

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Pi 0.0186
MarkerSeek score 64.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
672
variable_sites
18
pi
0.0186
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ATTAATCAACCCGAGGGACC 50.0 50.0 58.0 57.0 775-787 bp 1.0 74.9
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ATTAATCAACCCGAGGGACC 50.0 50.0 58.0 57.0 774-786 bp 1.0 74.8
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA TTAATCAACCCGAGGGACCC 50.0 55.0 58.0 59.1 774-786 bp 1.0 74.4
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC TTAATCAACCCGAGGGACCC 50.0 55.0 58.0 59.1 773-785 bp 1.0 74.3
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA ATTAATCAACCCGAGGGACC 50.0 50.0 58.2 57.0 829-841 bp 1.0 73.5