Hotspot

psbI-trnS-GCU

LSC · 7763-7912 · 150 bp

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Pi 0.0467

MarkerSeek score 79.00

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 150
variable_sites: 10
pi: 0.0467
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.1	58.0	226-247 bp	1.0	79.8
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.1	58.0	227-248 bp	1.0	79.8
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGA	TTTTTCGTACCGAGGGTTCG	50.0	50.0	57.1	58.2	189-210 bp	1.0	78.7
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGAA	TGGACTAAAGCGTCGGATTG	47.6	50.0	57.8	58.0	226-247 bp	1.0	77.5
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGAA	GTGGACTAAAGCGTCGGATT	47.6	50.0	57.8	58.0	227-248 bp	1.0	77.5