Hotspot

rpl16

LSC · 82968-84499 · 1532 bp

Back to Pritchardia

Pi 0

MarkerSeek score 59.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1532
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GTCCAGAATAAACCTGCGGT	AGGTTGAATAAAAATTCGATTGACCA	50.0	30.8	57.9	58.1	1628-1636 bp	1.0	40.4
rpl16_p2	GTCCAGAATAAACCTGCGGT	GGTTGAATAAAAATTCGATTGACCA	50.0	32.0	57.9	56.9	1627-1635 bp	1.0	40.4
rpl16_p3	GTCCAGAATAAACCTGCGGT	AGGTTGAATAAAAATTCGATTGACC	50.0	32.0	57.9	56.6	1628-1636 bp	1.0	40.4
rpl16_p4	TCCGTCCAGAATAAACCTGC	AGGTTGAATAAAAATTCGATTGACCA	50.0	30.8	57.6	58.1	1631-1639 bp	1.0	40.4
rpl16_p5	TCCGTCCAGAATAAACCTGC	GGTTGAATAAAAATTCGATTGACCA	50.0	32.0	57.6	56.9	1630-1638 bp	1.0	40.4