Hotspot

rps4-trnT-UGU

LSC · 46644-47027 · 384 bp

Back to Pritchardia

Pi 0

MarkerSeek score 56.50

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 384
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGTCCTGGTAAAGTCCCCAG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	455-509 bp	1.0	80.2
rps4-trnT-UGU_p2	AGTCCTGGTAAAGTCCCCAG	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.3	57.8	423-477 bp	1.0	79.3
rps4-trnT-UGU_p3	AGTCCTGGTAAAGTCCCCAG	CTGTTATGTGAGCCCGCTTA	55.0	50.0	58.3	57.7	476-530 bp	1.0	78.8
rps4-trnT-UGU_p4	AGTCCTGGTAAAGTCCCCAG	TGTTATGTGAGCCCGCTTAG	55.0	50.0	58.3	57.7	475-529 bp	1.0	78.8
rps4-trnT-UGU_p5	AGTCCTGGTAAAGTCCCCAG	CCGCTTAGCTCAGAGGTTAG	55.0	55.0	58.3	57.5	463-517 bp	1.0	77.3