Hotspot

atpI-rps2

LSC · 15399-15705 · 307 bp

Back to Pritchardia

Pi 0.01

MarkerSeek score 60.60

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 307
variable_sites: 3
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	GTTGGCCTACTTCTACACCC	TTGTGAGGGTCGTTCTACCT	55.0	50.0	57.6	58.0	413-420 bp	1.0	78.7
atpI-rps2_p2	GGCCTACTTCTACACCCGAT	TTGTGAGGGTCGTTCTACCT	55.0	50.0	58.6	58.0	410-417 bp	1.0	77.2
atpI-rps2_p3	GTTGGCCTACTTCTACACCC	TGCAATTTGTGAGGGTCGTT	55.0	45.0	57.6	58.3	419-426 bp	1.0	76.2
atpI-rps2_p4	GTTGGCCTACTTCTACACCC	GCAATTTGTGAGGGTCGTTC	55.0	50.0	57.6	57.7	418-425 bp	1.0	76.1
atpI-rps2_p5	GTTGGCCTACTTCTACACCC	TCTGCAATTTGTGAGGGTCG	55.0	50.0	57.6	58.5	421-428 bp	1.0	74.9