Hotspot

trnS-GCU-trnG-UCC

LSC · 8326-8976 · 651 bp

Back to Pritchardia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 651
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	CGGAATATGAAACTGAAAGACCC	45.0	43.5	58.0	57.5	797 bp	1.0	51.8
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	725 bp	1.0	51.4
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	ATGAAACTGAAAGACCCCTT	45.0	40.0	58.0	54.4	791 bp	1.0	51.1
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	728 bp	1.0	50.3
trnS-GCU-trnG-UCC_p5	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	727 bp	1.0	50.3