primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TTGCTCCATTCCTTCAACGA	GTGTGCTTGGGAGTTCTTGA	20	20	45.0	50.0	57.4	58.0	0.0	0.0	0.592379	120	1181	1151-1151	1.000000	75.3
psbA_p2	psbA	2	TTGCTCCATTCCTTCAACGA	ATACGTGTGCTTGGGAGTTC	20	20	45.0	50.0	57.4	57.9	0.0	0.0	0.654460	120	1181	1155-1155	1.000000	74.8
psbA_p3	psbA	3	TGCTCCATTCCTTCAACGAT	GTGTGCTTGGGAGTTCTTGA	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.820154	120	1181	1150-1150	1.000000	73.4
psbA_p4	psbA	4	TGCTCCATTCCTTCAACGAT	ATACGTGTGCTTGGGAGTTC	20	20	45.0	50.0	57.2	57.9	0.0	0.0	0.882235	120	1181	1154-1154	1.000000	72.9
psbA_p5	psbA	5	TGCTCCATTCCTTCAACGATT	GTGTGCTTGGGAGTTCTTGA	21	20	42.9	50.0	57.9	58.0	0.0	0.0	1.104555	120	1181	1150-1150	1.000000	71.2
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACTCCTTAAAGAGAAGCGGA	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	55.9	58.2	8.1	17.1	2.280619	3252	4064	916-916	1.000000	61.8
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACTCCTTAAAGAGAAGCGGA	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	55.9	58.2	8.1	17.1	2.280619	3252	4064	915-915	1.000000	61.8
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACTCCTTAAAGAGAAGCGGA	GGGTTGCTAACTCAACGGTA	20	20	45.0	50.0	55.9	57.5	8.1	0.0	2.572956	3252	4064	929-929	1.000000	59.4
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACTCCTTAAAGAGAAGCGGA	TTCGGGTTGCTAACTCAACG	20	20	45.0	50.0	55.9	58.5	8.1	0.0	2.607840	3252	4064	932-932	1.000000	59.1
matK-trnK-UUU_p5	matK-trnK-UUU	5	ACTCCTTAAAGAGAAGCGGAT	ACGGTAGAGTACTCGGCTTT	21	20	42.9	50.0	56.4	58.2	8.1	17.1	2.720297	3252	4064	915-915	1.000000	58.2
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCTGGACGAATCTGTTGC	GCTCAACCTACGGGAACTG	20	19	50.0	57.9	57.9	57.9	0.0	0.0	1.225083	4102	4812	882-882	1.000000	70.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	GTCTGGACGAATCTGTTGCT	GCTCAACCTACGGGAACTG	20	19	50.0	57.9	57.9	57.9	0.0	0.0	1.225083	4102	4812	881-881	1.000000	70.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AGTCTGGACGAATCTGTTGC	GTGCTCAACCTACGGGAAC	20	19	50.0	57.9	57.9	58.2	0.0	0.0	1.249110	4102	4812	884-884	1.000000	70.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GTCTGGACGAATCTGTTGCT	GTGCTCAACCTACGGGAAC	20	19	50.0	57.9	57.9	58.2	0.0	0.0	1.249110	4102	4812	883-883	1.000000	70.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	GCTCAACCTACGGGAACTG	20	19	50.0	57.9	58.2	57.9	20.3	0.0	1.307449	4102	4812	825-825	1.000000	69.5
rps16_p1	rps16	1	GCAAGAAAAAGGCAAATTGTGT	CCGGTTGAGCCAATGACTAT	22	20	36.4	50.0	57.1	57.7	0.0	0.0	3.212950	4813	5948	1309-1309	1.000000	54.3
rps16_p2	rps16	2	GCAAGAAAAAGGCAAATTGTGT	TATACACACACCGGTTGAGC	22	20	36.4	50.0	57.1	57.6	0.0	19.4	3.254427	4813	5948	1319-1319	1.000000	54.0
rps16_p3	rps16	3	AGCAAGAAAAAGGCAAATTGTG	CCGGTTGAGCCAATGACTAT	22	20	36.4	50.0	56.9	57.7	0.0	0.0	3.480732	4813	5948	1310-1310	1.000000	52.2
rps16_p4	rps16	4	AGCAAGAAAAAGGCAAATTGTG	TATACACACACCGGTTGAGC	22	20	36.4	50.0	56.9	57.6	0.0	19.4	3.522209	4813	5948	1320-1320	1.000000	51.8
rps16_p5	rps16	5	GCAAGAAAAAGGCAAATTGTGT	GGTATACACACACCGGTTGA	22	20	36.4	50.0	57.1	57.3	0.0	19.4	3.628283	4813	5948	1321-1321	1.000000	51.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.104521	5949	6979	1122-1122	1.000000	79.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.105939	5949	6979	1120-1120	1.000000	79.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AATCCACAGCCGATCATGTC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	5949	6979	1128-1128	1.000000	79.1
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GAATCCACAGCCGATCATGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	5949	6979	1129-1129	1.000000	79.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CAGCCGATCATGTCCTTCAA	GAAATTCGAAATGGGGCGTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.120355	5949	6979	1181-1181	1.000000	79.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	CGGAATATGAAACTGAAAGACCC	20	23	45.0	43.5	58.0	57.5	0.0	0.0	3.523869	8326	8976	797-797	1.000000	51.8
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	8326	8976	725-725	1.000000	51.4
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ATTAGCAATCCGCCGCTTTA	ATGAAACTGAAAGACCCCTT	20	20	45.0	40.0	58.0	54.4	0.0	0.0	3.617812	8326	8976	791-791	1.000000	51.1
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8326	8976	728-728	1.000000	50.3
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8326	8976	727-727	1.000000	50.3
trnG-UCC_p1	trnG-UCC	1	TGATTGCGCCTCACTCTTTT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	58.1	57.2	4.2	18.0	3.896810	8977	9752	917-917	1.000000	48.8
trnG-UCC_p2	trnG-UCC	2	TTGATTGCGCCTCACTCTTT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	58.1	57.2	4.2	18.0	3.896810	8977	9752	918-918	1.000000	48.8
trnG-UCC_p3	trnG-UCC	3	GATTGCGCCTCACTCTTTTG	TGCATATCCAAATTGATGATGCA	20	23	50.0	34.8	57.7	57.2	4.2	18.0	4.046422	8977	9752	916-916	1.000000	47.6
trnG-UCC_p4	trnG-UCC	4	ATCTTGATTGCGCCTCACTC	TGCATATCCAAATTGATGATGCA	20	23	50.0	34.8	58.3	57.2	4.2	18.0	4.131689	8977	9752	921-921	1.000000	46.9
trnG-UCC_p5	trnG-UCC	5	ATTGCGCCTCACTCTTTTGT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	58.4	57.2	4.2	18.0	4.183822	8977	9752	915-915	1.000000	46.5
atpI-rps2_p1	atpI-rps2	1	GTTGGCCTACTTCTACACCC	TTGTGAGGGTCGTTCTACCT	20	20	55.0	50.0	57.6	58.0	10.3	0.0	0.403622	15399	15705	413-420	1.000000	78.7
atpI-rps2_p2	atpI-rps2	2	GGCCTACTTCTACACCCGAT	TTGTGAGGGTCGTTCTACCT	20	20	55.0	50.0	58.6	58.0	0.0	0.0	0.594329	15399	15705	410-417	1.000000	77.2
atpI-rps2_p3	atpI-rps2	3	GTTGGCCTACTTCTACACCC	TGCAATTTGTGAGGGTCGTT	20	20	55.0	45.0	57.6	58.3	10.3	0.0	0.711897	15399	15705	419-426	1.000000	76.2
atpI-rps2_p4	atpI-rps2	4	GTTGGCCTACTTCTACACCC	GCAATTTGTGAGGGTCGTTC	20	20	55.0	50.0	57.6	57.7	10.3	0.0	0.730061	15399	15705	418-425	1.000000	76.1
atpI-rps2_p5	atpI-rps2	5	GTTGGCCTACTTCTACACCC	TCTGCAATTTGTGAGGGTCG	20	20	55.0	50.0	57.6	58.5	10.3	0.0	0.875584	15399	15705	421-428	1.000000	74.9
rpoC1_p1	rpoC1	1	TGAAACTGGAGAGACCCGAA	TTGCTCGTTCGAGAACTACG	20	20	50.0	50.0	58.3	58.0	0.0	23.5	0.328197	20966	23758	2953-2953	1.000000	77.4
rpoC1_p2	rpoC1	2	CTGGAGAGACCCGAATTCCT	TTGCTCGTTCGAGAACTACG	20	20	55.0	50.0	58.5	58.0	0.0	23.5	0.549679	20966	23758	2948-2948	1.000000	75.6
rpoC1_p3	rpoC1	3	TGAAACTGGAGAGACCCGAA	GCTCGTTCGAGAACTACGAT	20	20	50.0	50.0	58.3	57.5	0.0	23.5	0.743239	20966	23758	2951-2951	1.000000	74.1
rpoC1_p4	rpoC1	4	ACTGGAGAGACCCGAATTCC	TTGCTCGTTCGAGAACTACG	20	20	55.0	50.0	58.8	58.0	0.0	23.5	0.844241	20966	23758	2949-2949	1.000000	73.3
rpoC1_p5	rpoC1	5	TGGAGAGACCCGAATTCCTA	TTGCTCGTTCGAGAACTACG	20	20	50.0	50.0	57.2	58.0	0.0	23.5	0.880893	20966	23758	2947-2947	1.000000	73.0
petN-psbM_p1	petN-psbM	1	GTCTCACTTGGGCTGCTTTA	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.592428	29486	30322	993-993	1.000000	67.3
petN-psbM_p2	petN-psbM	2	ACTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	57.7	57.6	0.0	0.0	1.716560	29486	30322	988-988	1.000000	66.3
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	29486	30322	938-938	1.000000	66.0
petN-psbM_p4	petN-psbM	4	GTAAGTCTCACTTGGGCTGC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.6	57.6	0.0	0.0	1.963622	29486	30322	997-997	1.000000	64.4
petN-psbM_p5	petN-psbM	5	TAAGTCTCACTTGGGCTGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.6	57.6	0.0	0.0	2.055340	29486	30322	996-996	1.000000	63.6
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	GGATCCTAGTTCGGGACTGA	AATTCTTCCTGGGTCGATGC	20	20	55.0	50.0	57.9	58.0	3.8	0.0	0.112957	31518	31900	562-562	1.000000	79.7
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	GTGCTCTGACCGATTGAACT	AATTCTTCCTGGGTCGATGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.130177	31518	31900	505-505	1.000000	79.6
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	GGATCCTAGTTCGGGACTGA	GGGGACGGACTGTAAATTCG	20	20	55.0	55.0	57.9	58.4	3.8	0.0	0.420330	31518	31900	529-529	1.000000	77.2
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	ATCGGATCCTAGTTCGGGAC	AATTCTTCCTGGGTCGATGC	20	20	55.0	50.0	58.4	58.0	10.8	0.0	0.425635	31518	31900	565-565	1.000000	77.2
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CATCGGATCCTAGTTCGGGA	AATTCTTCCTGGGTCGATGC	20	20	55.0	50.0	58.4	58.0	10.8	0.0	0.426800	31518	31900	566-566	1.000000	77.2
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	AGTCCTGGTAAAGTCCCCAG	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.3	57.9	0.0	0.0	0.474936	46644	47027	455-509	1.000000	80.2
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	AGTCCTGGTAAAGTCCCCAG	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	58.3	57.8	0.0	0.0	0.582690	46644	47027	423-477	1.000000	79.3
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	AGTCCTGGTAAAGTCCCCAG	CTGTTATGTGAGCCCGCTTA	20	20	55.0	50.0	58.3	57.7	0.0	0.0	0.651724	46644	47027	476-530	1.000000	78.8
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	AGTCCTGGTAAAGTCCCCAG	TGTTATGTGAGCCCGCTTAG	20	20	55.0	50.0	58.3	57.7	0.0	0.0	0.651724	46644	47027	475-529	1.000000	78.8
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	AGTCCTGGTAAAGTCCCCAG	CCGCTTAGCTCAGAGGTTAG	20	20	55.0	55.0	58.3	57.5	0.0	7.6	0.842705	46644	47027	463-517	1.000000	77.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47101	48064	1008-1014	1.000000	79.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47101	48064	1016-1022	1.000000	78.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47101	48064	1040-1046	1.000000	78.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	47101	48064	1048-1054	1.000000	78.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	47101	48064	1031-1037	1.000000	77.6
rpl16_p1	rpl16	1	GTCCAGAATAAACCTGCGGT	AGGTTGAATAAAAATTCGATTGACCA	20	26	50.0	30.8	57.9	58.1	0.0	0.0	6.217863	82968	84499	1628-1636	1.000000	40.4
rpl16_p2	rpl16	2	GTCCAGAATAAACCTGCGGT	GGTTGAATAAAAATTCGATTGACCA	20	25	50.0	32.0	57.9	56.9	0.0	0.0	6.223742	82968	84499	1627-1635	1.000000	40.4
rpl16_p3	rpl16	3	GTCCAGAATAAACCTGCGGT	AGGTTGAATAAAAATTCGATTGACC	20	25	50.0	32.0	57.9	56.6	0.0	0.0	6.464149	82968	84499	1628-1636	1.000000	40.4
rpl16_p4	rpl16	4	TCCGTCCAGAATAAACCTGC	AGGTTGAATAAAAATTCGATTGACCA	20	26	50.0	30.8	57.6	58.1	0.0	0.0	6.505028	82968	84499	1631-1639	1.000000	40.4
rpl16_p5	rpl16	5	TCCGTCCAGAATAAACCTGC	GGTTGAATAAAAATTCGATTGACCA	20	25	50.0	32.0	57.6	56.9	0.0	0.0	6.510907	82968	84499	1630-1638	1.000000	40.4
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	119683	120223	735-857	1.000000	76.4
psaC-ndhE_p2	psaC-ndhE	2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	119683	120223	734-856	1.000000	76.4
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	20	20	50.0	50.0	58.1	58.6	0.0	12.2	0.757229	119683	120223	755-877	1.000000	76.3
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	58.1	58.8	0.0	6.0	0.891951	119683	120223	733-855	1.000000	75.3
psaC-ndhE_p5	psaC-ndhE	5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.943571	119683	120223	710-832	1.000000	74.9
ndhI-ndhA_p1	ndhI-ndhA	1	ACCTTGCAGCTCGTATTGTT	TGACAACTTCTTCCCAACTTGT	20	22	45.0	40.9	57.8	58.0	0.0	0.0	2.221875	121958	122060	181-196	1.000000	66.2
ndhI-ndhA_p2	ndhI-ndhA	2	ACCTTGCAGCTCGTATTGTT	TGCCTAGGATAAGAATGGACCA	20	22	45.0	45.5	57.8	58.3	0.0	14.8	2.520684	121958	122060	259-274	1.000000	63.8
ndhI-ndhA_p3	ndhI-ndhA	3	ACCTTGCAGCTCGTATTGTT	AGATGGACTTTGCCTAGGAT	20	20	45.0	45.0	57.8	55.6	0.0	14.8	2.602740	121958	122060	269-284	1.000000	63.2
ndhI-ndhA_p4	ndhI-ndhA	4	ACCTTGCAGCTCGTATTGTT	GCCTAGGATAAGAATGGACCA	20	21	45.0	47.6	57.8	56.6	0.0	14.8	2.612877	121958	122060	258-273	1.000000	63.1
ndhI-ndhA_p5	ndhI-ndhA	5	ACCTTGCAGCTCGTATTGTT	TGCCTAGGATAAGAATGGACC	20	21	45.0	47.6	57.8	56.6	0.0	14.8	2.612877	121958	122060	259-274	1.000000	63.1