Hotspot

ndhF-rpl32

SSC · 116112-117038 · 927 bp

Back to Pristimera

Pi 0.0411

MarkerSeek score 76.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 927
variable_sites: 54
pi: 0.0411
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACATCCCAACCAATTCCC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	2088-2106 bp	1.0	86.3
ndhF-rpl32_p2	ACACATCCCAACCAATTCCC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	2089-2107 bp	1.0	86.3
ndhF-rpl32_p3	CACATCCCAACCAATTCCCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	2087-2105 bp	1.0	86.3
ndhF-rpl32_p4	CACATCCCAACCAATTCCCA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	2088-2106 bp	1.0	86.3
ndhF-rpl32_p5	ACACATCCCAACCAATTCCC	CTGTAGCAAGGGATTTCGCT	50.0	50.0	58.1	58.0	1510-1529 bp	1.0	86.1