Hotspot

trnN-GUU-ndhF

IRb · 112469-114620 · 2152 bp

Back to Pristimera

Pi 0.0281

MarkerSeek score 66.80

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 2152
variable_sites: 73
pi: 0.0281
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	ATCTCCCCAGGTAGGATTCG	CTTGTTACACGGCCGGATTA	55.0	50.0	58.0	58.0	2256-2466 bp	1.0	86.3
trnN-GUU-ndhF_p2	CGGGAGAATCAATGACTGCA	CTTGTTACACGGCCGGATTA	50.0	50.0	58.0	58.0	2526-2737 bp	1.0	86.2
trnN-GUU-ndhF_p3	GGCAGAATAGCAGAGCAAGT	CTTGTTACACGGCCGGATTA	50.0	50.0	58.0	58.0	2610-2813 bp	1.0	86.1
trnN-GUU-ndhF_p4	ATCTCCCCAGGTAGGATTCG	GCTTTAGGTCTGGGCTCTTC	55.0	55.0	58.0	58.0	2563-2773 bp	1.0	85.9
trnN-GUU-ndhF_p5	AAGACAGAGGGAACGGGTTA	CTTGTTACACGGCCGGATTA	50.0	50.0	58.0	58.0	2496 bp	0.333333	53.1