Hotspot

rps12-clpP

LSC · 72748-72956 · 209 bp

Back to Pristimera

Pi 0.0408

MarkerSeek score 69.30

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 209
variable_sites: 12
pi: 0.0408
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GCTGCCTTGTATTCCTAATAAGT	TTGTTGATCGTGTAGCGGTT	39.1	45.0	56.4	57.8	271-278 bp	1.0	47.4
rps12-clpP_p2	GCTGCCTTGTATTCCTAATAAGT	TATGTCAGCAACAGAAGCCC	39.1	50.0	56.4	57.9	303-310 bp	1.0	47.2
rps12-clpP_p3	GCTGCCTTGTATTCCTAATAAGT	GTTGATCGTGTAGCGGTTGA	39.1	50.0	56.4	58.3	269-276 bp	1.0	46.4
rps12-clpP_p4	GCTGCCTTGTATTCCTAATAAGT	GGCAAACCCTTATGGATGGT	39.1	50.0	56.4	57.8	350-357 bp	1.0	46.2
rps12-clpP_p5	AAGTTGTTTAAGAGTTGGCA	TATGTCAGCAACAGAAGCCC	35.0	50.0	53.2	57.9	284-291 bp	1.0	46.2