Hotspot

trnT-UGU-trnL-UAA

LSC · 49130-50199 · 1070 bp

Back to Pristimera

Pi 0.0294

MarkerSeek score 68.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1070
variable_sites: 45
pi: 0.0294
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CTCTAACCGCTGAGCTAAGC	CCGTAGCGTCTACCAATTCC	55.0	55.0	58.2	58.2	1134-1165 bp	1.0	83.4
trnT-UGU-trnL-UAA_p2	CTCTAACCGCTGAGCTAAGC	AGTCCGTAGCGTCTACCAAT	55.0	50.0	58.2	58.2	1137-1168 bp	1.0	82.7
trnT-UGU-trnL-UAA_p3	CTCTAACCGCTGAGCTAAGC	ATTCCGCCATATCCCCCATA	55.0	50.0	58.2	58.0	1119 bp	0.333333	51.9
trnT-UGU-trnL-UAA_p4	CTCTAACCGCTGAGCTAAGC	TTCCGCCATATCCCCCATAT	55.0	50.0	58.2	58.0	1118 bp	0.333333	51.9
trnT-UGU-trnL-UAA_p5	CAAATGCGATGCTCTAACCG	TTCCGCCATATCCCCCATAT	50.0	50.0	57.6	58.0	1129 bp	0.333333	50.2