Hotspot

psbZ-trnG-GCC

LSC · 37938-38734 · 797 bp

Back to Pristimera

Pi 0.0483

MarkerSeek score 75.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 48
pi: 0.0483
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CAAGACCGGAGCTATCAACC	CGACGTTGTTTTTCGACTGG	55.0	50.0	58.1	58.0	1908-2010 bp	1.0	86.5
psbZ-trnG-GCC_p2	CAAGACCGGAGCTATCAACC	CTTGAGGTCACGGGTTCAAA	55.0	50.0	58.1	58.0	1582-1684 bp	1.0	86.5
psbZ-trnG-GCC_p3	ATTCGCCAAGCAAAAGGAGA	CGACGTTGTTTTTCGACTGG	45.0	50.0	58.1	58.0	1979-2081 bp	1.0	86.2
psbZ-trnG-GCC_p4	CAAGACCGGAGCTATCAACC	CGAGACTTTGGGTTATCCGG	55.0	55.0	58.1	58.1	1869-1971 bp	1.0	86.0
psbZ-trnG-GCC_p5	CCCATGCCCGAATCGAATAA	CGACGTTGTTTTTCGACTGG	50.0	50.0	58.1	58.0	1764 bp	0.333333	52.5