Hotspot

trnT-GGU-psbD

LSC · 33086-34580 · 1495 bp

Back to Pristimera

Pi 0.0259
MarkerSeek score 68.20
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1495
variable_sites
50
pi
0.0259
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CTCCGTAACCAGTCATCCAT 55.0 50.0 58.1 56.7 1279-1627 bp 1.0 75.9
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA CTCCGTAACCAGTCATCCAT 50.0 50.0 58.1 56.7 1282-1630 bp 1.0 75.7
trnT-GGU-psbD_p3 GGCCCTTTTAACTCAGTGGT CTCCGTAACCAGTCATCCAT 50.0 50.0 57.7 56.7 1294-1642 bp 1.0 74.1
trnT-GGU-psbD_p4 TGGTAGAGTAACGCCATGGT CTCCGTAACCAGTCATCCAT 50.0 50.0 58.4 56.7 1278-1626 bp 1.0 72.9
trnT-GGU-psbD_p5 CTCAGTGGTAGAGTAACGCC CTCCGTAACCAGTCATCCAT 55.0 50.0 57.4 56.7 1283-1631 bp 1.0 71.9