Hotspot

trnS-GCU-trnG-UCC

LSC · 8128-8971 · 844 bp

Back to Pristimera

Pi 0.03
MarkerSeek score 70.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
844
variable_sites
37
pi
0.03
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 913-977 bp 1.0 57.8
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 912-976 bp 1.0 57.8
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 915-979 bp 1.0 56.8
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 916-980 bp 1.0 56.7
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 914-978 bp 1.0 56.7