Hotspot

psbI-trnS-GCU

LSC · 7811-8039 · 229 bp

Back to Pristimera

Pi 0.0393

MarkerSeek score 68.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 229
variable_sites: 12
pi: 0.0393
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTTTGGCAAGCTGCTGTTAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	897-927 bp	1.0	86.6
psbI-trnS-GCU_p2	GTTTGGCAAGCTGCTGTTAG	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.9	58.0	898-928 bp	1.0	86.6
psbI-trnS-GCU_p3	GTTTGGCAAGCTGCTGTTAG	GTTCTTCGTACCGAGGGTTC	50.0	55.0	57.9	58.0	861-891 bp	1.0	86.6
psbI-trnS-GCU_p4	GTTTGGCAAGCTGCTGTTAG	AGTTTGTGTTGACCGATGCT	50.0	45.0	57.9	58.0	1586 bp	0.333333	52.6
psbI-trnS-GCU_p5	GTTTGGCAAGCTGCTGTTAG	TGCTACTGGCCAAAACTGTT	50.0	45.0	57.9	57.9	1570 bp	0.333333	52.4