Hotspot

trnK-UUU-trnQ-UUG

LSC · 4644-6613 · 1970 bp

Back to Pristimera

Pi 0.0291

MarkerSeek score 66.10

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1970
variable_sites: 79
pi: 0.0291
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	CGCGATCTTCCCAACTCTAC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.2	58.1	2006-2108 bp	1.0	85.3
trnK-UUU-trnQ-UUG_p2	CGCGATCTTCCCAACTCTAC	CTAACAGCAGCTTGCCAAAC	55.0	50.0	58.2	57.9	2616-2682 bp	1.0	85.2
trnK-UUU-trnQ-UUG_p3	AAAGCCGAGTACTCTACCGT	CTAACAGCAGCTTGCCAAAC	50.0	50.0	58.2	57.9	2536-2602 bp	0.666667	71.0
trnK-UUU-trnQ-UUG_p4	AAGCCGAGTACTCTACCGTT	CTAACAGCAGCTTGCCAAAC	50.0	50.0	58.2	57.9	2535-2601 bp	0.666667	71.0
trnK-UUU-trnQ-UUG_p5	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1925-2027 bp	0.666667	70.9