Hotspot

ndhG-ndhI

SSC · 124848-125415 · 568 bp

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Pi 0.0661

MarkerSeek score 76.00

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 568
variable_sites: 32
pi: 0.0661
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CAAGAACCAGTCCCAACGAA	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.0	57.9	703-785 bp	1.0	86.7
ndhG-ndhI_p2	TCCCAACGAAAAGGCAGAAA	AATCAAATTGCTTTGGGCCG	45.0	45.0	57.9	57.9	693-775 bp	1.0	86.6
ndhG-ndhI_p3	GATTTATTACTGGCGGGCCA	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.0	57.9	1110-1192 bp	1.0	85.9
ndhG-ndhI_p4	CAAGAACCAGTCCCAACGAA	TGGTCAACAAACCGTACGAG	50.0	50.0	58.0	58.1	1082-1164 bp	1.0	85.3
ndhG-ndhI_p5	GATTTATTACTGGCGGGCCA	TGGTCAACAAACCGTACGAG	50.0	50.0	58.0	58.1	1489-1571 bp	1.0	85.0