Hotspot

ndhD-psaC

SSC · 123122-123248 · 127 bp

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Pi 0.112

MarkerSeek score 78.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 127
variable_sites: 14
pi: 0.112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TAAGGAACCCGCGGATATTG	TGTGAATCTGCCTGTCCAAC	50.0	50.0	57.5	58.1	272-274 bp	1.0	79.6
ndhD-psaC_p2	GATTAAGGAACCCGCGGATA	TGTGAATCTGCCTGTCCAAC	50.0	50.0	57.2	58.1	275-277 bp	1.0	77.2
ndhD-psaC_p3	TAAGGAACCCGCGGATATTG	GATGTGAATCTGCCTGTCCA	50.0	50.0	57.5	57.6	274-276 bp	1.0	77.1
ndhD-psaC_p4	TAAGGAACCCGCGGATATTG	GAGGATTGTGTCGGCTGTAA	50.0	50.0	57.5	57.6	296-298 bp	1.0	77.0
ndhD-psaC_p5	AGATTAAGGAACCCGCGGAT	TGTGAATCTGCCTGTCCAAC	50.0	50.0	58.9	58.1	276-278 bp	1.0	76.8