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petA-psbJ

LSC · 67386-68149 · 764 bp

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Pi 0.0414

MarkerSeek score 79.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 31
pi: 0.0414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	901-958 bp	1.0	79.1
petA-psbJ_p2	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	906-963 bp	1.0	78.6
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.4	57.6	910-967 bp	1.0	78.1
petA-psbJ_p4	ACGTGTCCAAGGTCTCTTGT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.9	57.6	902-959 bp	1.0	74.0
petA-psbJ_p5	ATCCGTTACGTGTCCAAGGT	ATTCCGGATTGGGTTCATCC	50.0	50.0	59.0	57.6	909-966 bp	1.0	72.8