Hotspot

accD-psaI

LSC · 63652-64230 · 579 bp

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Pi 0.0455

MarkerSeek score 76.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 579
variable_sites: 25
pi: 0.0455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGGGCGTTCTGAGTGAGTTA	AGGTAAGTTGCTGAGAGTTGTC	50.0	45.5	58.4	58.0	658-671 bp	1.0	67.6
accD-psaI_p2	AGGGCGTTCTGAGTGAGTTA	AGGTAAGTTGCTGAGAGTTGT	50.0	42.9	58.4	56.9	658-671 bp	1.0	66.6
accD-psaI_p3	AGGGCGTTCTGAGTGAGTTA	ACAGAAGGTAAGTTGCTGAGAG	50.0	45.5	58.4	57.7	663-676 bp	1.0	65.5
accD-psaI_p4	AGGGCGTTCTGAGTGAGTTA	CAGAAGGTAAGTTGCTGAGAGT	50.0	45.5	58.4	57.7	662-675 bp	1.0	65.5
accD-psaI_p5	AGGGCGTTCTGAGTGAGTTA	GGTAAGTTGCTGAGAGTTGTCA	50.0	45.5	58.4	58.3	657-670 bp	1.0	65.5