Hotspot

trnM-CAU-atpE

LSC · 56830-57130 · 301 bp

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Pi 0.105

MarkerSeek score 85.80

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 301
variable_sites: 29
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCAGGAGTCATTGGTTCAA	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.1	58.0	362-386 bp	1.0	89.2
trnM-CAU-atpE_p2	GGCAGGAGTCATTGGTTCAA	CGAGCTAGGACACGAGTAGA	50.0	55.0	58.1	58.1	354-378 bp	1.0	89.1
trnM-CAU-atpE_p3	TATTTTGGGTCCCTCGCTTG	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.9	58.0	442-466 bp	1.0	87.9
trnM-CAU-atpE_p4	TATTTTGGGTCCCTCGCTTG	CGAGCTAGGACACGAGTAGA	50.0	55.0	57.9	58.1	434-458 bp	1.0	87.7
trnM-CAU-atpE_p5	GTCCCTCGCTTGTTTCTCTT	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.8	58.0	434-458 bp	1.0	87.6