Hotspot

rps4-trnT-UGU

LSC · 50035-50757 · 723 bp

Back to Prioria

Pi 0.0982
MarkerSeek score 88.70
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
723
variable_sites
60
pi
0.0982
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
88.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 CGGAGCGTGATTGGTTTCTA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 802-901 bp 1.0 88.5
rps4-trnT-UGU_p2 TAAGATCGCTTCCGGCTCTA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 784-883 bp 1.0 88.5
rps4-trnT-UGU_p3 TTTCTAAGATCGCTTCCGGC CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 788-887 bp 1.0 88.2
rps4-trnT-UGU_p4 CGGAGCGTGATTGGTTTCTA ATGGTCATCGGTTCGATTCC 50.0 50.0 58.0 57.8 770-869 bp 1.0 87.9
rps4-trnT-UGU_p5 TAAGATCGCTTCCGGCTCTA ATGGTCATCGGTTCGATTCC 50.0 50.0 58.0 57.8 752-851 bp 1.0 87.9