Hotspot

trnG-UCC-trnfM-CAU

LSC · 40674-40922 · 249 bp

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Pi 0.174

MarkerSeek score 83.20

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 249
variable_sites: 41
pi: 0.174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	324-327 bp	1.0	89.8
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	322-325 bp	1.0	88.7
trnG-UCC-trnfM-CAU_p3	GGGTTCAATTCCCGCTATCC	CTTGAGGTCACGGGTTCAAA	55.0	50.0	58.4	58.0	303-306 bp	1.0	88.2
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	345-348 bp	1.0	88.1
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	344-347 bp	1.0	88.1