Hotspot

psbZ-trnG-UCC

LSC · 39786-40602 · 817 bp

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Pi 0.113

MarkerSeek score 87.90

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 84
pi: 0.113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 87.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GATGGTTGGTCGAGTAACAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	55.4	57.8	963-987 bp	1.0	68.8
psbZ-trnG-UCC_p2	GATGGTTGGTCGAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	42.9	50.0	56.2	57.8	963-987 bp	1.0	67.1
psbZ-trnG-UCC_p3	GATGGTTGGTCGAGTAACAA	GGATAGCGGGAATTGAACCC	45.0	55.0	55.4	58.4	984-1008 bp	1.0	66.9
psbZ-trnG-UCC_p4	TGGTTGGTCGAGTAACAAAA	CGTCTTCTCCTTGGCAAAGA	40.0	50.0	55.1	57.8	961-985 bp	1.0	66.7
psbZ-trnG-UCC_p5	GATGGTTGGTCGAGTAACAA	AATTGAACCCGCGTCTTCTC	45.0	50.0	55.4	58.6	974-998 bp	1.0	65.6