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psbI-trnS-GCU

LSC · 8075-8361 · 287 bp

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Pi 0.0563

MarkerSeek score 75.70

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 287
variable_sites: 16
pi: 0.0563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TCCTATCTAACGATCCGGGAC	ATTTTCGTACCGAGGGTTCG	52.4	50.0	58.2	58.0	367-554 bp	1.0	73.6
psbI-trnS-GCU_p2	TCCTATCTAACGATCCGGGAC	TGGACTAAAGCGTCGGATTG	52.4	50.0	58.2	58.0	404-591 bp	1.0	73.3
psbI-trnS-GCU_p3	TCCTATCTAACGATCCGGGAC	GTGGACTAAAGCGTCGGATT	52.4	50.0	58.2	58.0	405-592 bp	1.0	73.3
psbI-trnS-GCU_p4	TCCTATCTAACGATCCGGGA	ATTTTCGTACCGAGGGTTCG	50.0	50.0	56.7	58.0	367-554 bp	1.0	73.2
psbI-trnS-GCU_p5	TCCTATCTAACGATCCGGGA	TGGACTAAAGCGTCGGATTG	50.0	50.0	56.7	58.0	404-591 bp	1.0	72.9