Hotspot

rpl2

IRa · 157321-158809 · 1489 bp

Back to Prinsepia

Pi 0.00179

MarkerSeek score 45.60

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1489
variable_sites: 4
pi: 0.00179
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GAATGGTTAAAGCGCCCAAC	TATAATGGTAGACGCCCGGG	50.0	55.0	58.0	58.7	2208-2209 bp	1.0	74.4
rpl2_p2	AGCATTGGGTCGAACTCTTC	TATAATGGTAGACGCCCGGG	50.0	55.0	57.9	58.7	1875-1876 bp	1.0	73.7
rpl2_p3	GCATTGGGTCGAACTCTTCT	TATAATGGTAGACGCCCGGG	50.0	55.0	57.9	58.7	1874-1875 bp	1.0	73.7
rpl2_p4	CGCTTCAACCGGGTTATTCT	TATAATGGTAGACGCCCGGG	50.0	55.0	58.3	58.7	1749-1750 bp	1.0	72.4
rpl2_p5	GCTAAGCATCCATGGCTGAA	TATAATGGTAGACGCCCGGG	50.0	55.0	58.3	58.7	2225-2226 bp	1.0	71.9