Hotspot

rps15-ycf1

SSC · 127781-128187 · 407 bp

Back to Prinsepia

Pi 0.0411

MarkerSeek score 73.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 407
variable_sites: 24
pi: 0.0411
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGTGAAATAACCGAGATGAATGA	TGTATCCGCGATTGAAAATGA	34.8	38.1	56.0	56.1	433-468 bp	1.0	48.2
rps15-ycf1_p2	TGTGAAATAACCGAGATGAATGA	TGTATCCGCGATTGAAAATGAA	34.8	36.4	56.0	56.8	433-468 bp	1.0	48.2
rps15-ycf1_p3	TGTGAAATAACCGAGATGAATGAA	TGTATCCGCGATTGAAAATGA	33.3	38.1	56.7	56.1	433-468 bp	1.0	48.2
rps15-ycf1_p4	TTGTGAAATAACCGAGATGAATGA	TGTATCCGCGATTGAAAATGA	33.3	38.1	56.7	56.1	434-469 bp	1.0	48.2
rps15-ycf1_p5	TGTGAAATAACCGAGATGAATG	TGTATCCGCGATTGAAAATGA	36.4	38.1	54.6	56.1	433-468 bp	1.0	48.2