Hotspot

rpl32-trnL-UAG

SSC · 116860-118393 · 1534 bp

Back to Prinsepia

Pi 0.0293

MarkerSeek score 67.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1534
variable_sites: 66
pi: 0.0293
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGAGGTGGGTTGGATTATT	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	1469-2053 bp	1.0	87.0
rpl32-trnL-UAG_p2	GCGAGGTGGGTTGGATTATT	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	1470-2054 bp	1.0	87.0
rpl32-trnL-UAG_p3	GGAAAGGATATTGGGCAGCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	1161-1709 bp	1.0	86.9
rpl32-trnL-UAG_p4	GCGAGGTGGGTTGGATTATT	ATCCACACAAAAGAGCTGCA	50.0	45.0	57.9	58.0	2063-2654 bp	1.0	86.4
rpl32-trnL-UAG_p5	GCGAGGTGGGTTGGATTATT	TCCACACAAAAGAGCTGCAT	50.0	45.0	57.9	58.0	2062-2653 bp	1.0	86.4