Hotspot

ndhF-rpl32

SSC · 115755-116658 · 904 bp

Back to Prinsepia

Pi 0.0453

MarkerSeek score 77.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 904
variable_sites: 59
pi: 0.0453
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCCGTTGGAAAAAGT	GGTTGGATGTGAAAGACA	40.0	44.4	57.0	52.0	836-1033 bp	1.0	48.9
ndhF-rpl32_p2	TTTGTTGCCGTTGGAAAAAGT	GGTTGGATGTGAAAGACA	38.1	44.4	57.7	52.0	837-1034 bp	1.0	48.9
ndhF-rpl32_p3	TGTTGCCGTTGGAAAAAGTAG	GGTTGGATGTGAAAGACA	42.9	44.4	57.3	52.0	835-1032 bp	1.0	48.9
ndhF-rpl32_p4	TGTTGCCGTTGGAAAAAGTA	GGTTGGATGTGAAAGACA	40.0	44.4	56.1	52.0	835-1032 bp	1.0	48.9
ndhF-rpl32_p5	GTTGCCGTTGGAAAAAGTAGA	GGTTGGATGTGAAAGACA	42.9	44.4	57.0	52.0	834-1031 bp	1.0	48.9