Hotspot

psaJ-rpl33

LSC · 70072-70535 · 464 bp

Back to Prinsepia

Pi 0.0327

MarkerSeek score 72.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 22
pi: 0.0327
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCGGATGCGTTGATATTCC	ACACGATTTCGAACACAGCT	50.0	45.0	57.8	58.1	568-569 bp	1.0	83.0
psaJ-rpl33_p2	TCCGGATGCGTTGATATTCC	ACGATTTCGAACACAGCTGT	50.0	45.0	57.8	58.1	566-567 bp	1.0	83.0
psaJ-rpl33_p3	GTTCGGGTCTTTAGCAGGTT	ACGATTTCGAACACAGCTGT	50.0	45.0	57.8	58.1	611-612 bp	1.0	82.5
psaJ-rpl33_p4	GTTCGGGTCTTTAGCAGGTT	ACACGATTTCGAACACAGCT	50.0	45.0	57.8	58.1	613-614 bp	1.0	82.4
psaJ-rpl33_p5	TGGCACCGGTAGTAAGTACT	ACGATTTCGAACACAGCTGT	50.0	45.0	57.8	58.1	636-637 bp	1.0	81.9