Hotspot

accD-psaI

LSC · 61757-62537 · 781 bp

Back to Prinsepia

Pi 0.029

MarkerSeek score 67.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 33
pi: 0.029
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CTGAGTGAGTTGTTTCGGCT	CGCTGTTGAGAGTTATCAT	50.0	42.1	58.1	52.5	824-864 bp	1.0	45.8
accD-psaI_p2	TCTGAGTGAGTTGTTTCGGC	CGCTGTTGAGAGTTATCAT	50.0	42.1	58.1	52.5	825-865 bp	1.0	45.8
accD-psaI_p3	AGTTGTTTCGGCTACATGCT	CGCTGTTGAGAGTTATCAT	45.0	42.1	57.8	52.5	817-857 bp	1.0	45.8
accD-psaI_p4	GAGTTGTTTCGGCTACATGC	CGCTGTTGAGAGTTATCAT	50.0	42.1	57.5	52.5	818-858 bp	1.0	45.8
accD-psaI_p5	GGCGTTCTGAGTGAGTTGTT	CGCTGTTGAGAGTTATCAT	50.0	42.1	58.4	52.5	830-870 bp	1.0	45.7