Hotspot

ndhC-trnV-UAC

LSC · 53872-54356 · 485 bp

Back to Prinsepia

Pi 0.0363
MarkerSeek score 72.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
485
variable_sites
25
pi
0.0363
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ACTATCAGAAATGCCCAGAA AGAAGGTCTACGGTTCGAGT 40.0 50.0 54.0 57.8 546-580 bp 1.0 54.0
ndhC-trnV-UAC_p2 ACTATCAGAAATGCCCAGAA GCACGTTGGGTCTTTGAAAC 40.0 50.0 54.0 58.2 610-644 bp 1.0 53.9
ndhC-trnV-UAC_p3 ACTATCAGAAATGCCCAGAAA GCACGTTGGGTCTTTGAAAC 38.1 50.0 54.9 58.2 610-644 bp 1.0 53.0
ndhC-trnV-UAC_p4 ACTATCAGAAATGCCCAGAA AATGCACGTTGGGTCTTTGA 40.0 45.0 54.0 58.3 613-647 bp 1.0 52.7
ndhC-trnV-UAC_p5 ACTATCAGAAATGCCCAGAA ATGCACGTTGGGTCTTTGAA 40.0 45.0 54.0 58.3 612-646 bp 1.0 52.7