Hotspot

trnC-GCA-petN

LSC · 29031-29985 · 955 bp

Back to Prinsepia

Pi 0.029

MarkerSeek score 68.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 955
variable_sites: 40
pi: 0.029
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCATTCCGACGAAGAAGTC	50.0	50.0	58.1	58.0	1517-1846 bp	0.666667	66.7
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	GAGCATTCCGACGAAGAAGT	50.0	50.0	58.1	58.0	1518-1847 bp	0.666667	66.7
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	TGAGCATTCCGACGAAGAAG	50.0	50.0	58.1	58.0	1519-1848 bp	0.666667	66.7
trnC-GCA-petN_p4	ATGCTCCCTCCCCGTTATTA	AGCATTCCGACGAAGAAGTC	50.0	50.0	57.9	58.0	2179-2510 bp	0.666667	66.4
trnC-GCA-petN_p5	ATGCTCCCTCCCCGTTATTA	GAGCATTCCGACGAAGAAGT	50.0	50.0	57.9	58.0	2180-2511 bp	0.666667	66.4