Hotspot

atpA-atpF

LSC · 12228-12284 · 57 bp

Back to Prinsepia

Pi 0.0247

MarkerSeek score 72.50

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 57
variable_sites: 2
pi: 0.0247
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CTCAATACGCTCGCGGATAA	CAGCAAGCTTTACAAGGAGC	50.0	50.0	58.0	57.4	228-231 bp	1.0	76.4
atpA-atpF_p2	CCGTCGCCTACTTGAAGTAC	CAGCAAGCTTTACAAGGAGC	55.0	50.0	58.1	57.4	287-290 bp	1.0	75.6
atpA-atpF_p3	CGTCGCCTACTTGAAGTACA	CAGCAAGCTTTACAAGGAGC	50.0	50.0	57.4	57.4	286-289 bp	1.0	71.4
atpA-atpF_p4	TCAATACGCTCGCGGATAAT	CAGCAAGCTTTACAAGGAGC	45.0	50.0	57.3	57.4	227-230 bp	1.0	71.0
atpA-atpF_p5	TATTGCTCAATACGCTCGCG	CAGCAAGCTTTACAAGGAGC	50.0	50.0	58.9	57.4	233-236 bp	1.0	69.7