Hotspot

trnG-UCC-trnR-UCU

LSC · 10245-10513 · 269 bp

Back to Prinsepia

Pi 0.0484

MarkerSeek score 67.80

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 269
variable_sites: 17
pi: 0.0484
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	CTAACGATGCGGGTTCGATT	TGTCCTATCCATTAGACAATG	50.0	38.1	58.4	52.4	194-328 bp	1.0	49.0
trnG-UCC-trnR-UCU_p2	AGCCTTCCAAGCTAACGATG	TGTCCTATCCATTAGACAATG	50.0	38.1	58.0	52.4	205-339 bp	1.0	48.8
trnG-UCC-trnR-UCU_p3	CCTAGCCTTCCAAGCTAACG	TGTCCTATCCATTAGACAATG	55.0	38.1	58.1	52.4	208-342 bp	1.0	48.7
trnG-UCC-trnR-UCU_p4	CCCTAGCCTTCCAAGCTAAC	TGTCCTATCCATTAGACAATG	55.0	38.1	57.7	52.4	209-343 bp	1.0	48.7
trnG-UCC-trnR-UCU_p5	ACCAAAGTTTCACGTTCGGT	TGTCCTATCCATTAGACAATG	45.0	38.1	58.3	52.4	275-409 bp	1.0	47.8