Hotspot

psbI-trnS-GCU

LSC · 8592-8720 · 129 bp

Back to Prinsepia

Pi 0.0362

MarkerSeek score 69.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 129
variable_sites: 7
pi: 0.0362
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.1	58.0	226-231 bp	1.0	75.0
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.1	58.0	227-232 bp	1.0	74.9
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGA	CAATTGGGAGGAGAGATGGC	50.0	55.0	57.1	58.0	250-255 bp	1.0	74.6
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGA	AATTGGGAGGAGAGATGGCT	50.0	50.0	57.1	58.1	249-254 bp	1.0	74.0
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGAA	TGGACTAAAGCGTCGGATTG	47.6	50.0	57.8	58.0	226-231 bp	1.0	72.6