Hotspot

trnK-UUU-rps16

LSC · 4455-5555 · 1101 bp

Back to Prinsepia

Pi 0.0242
MarkerSeek score 70.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1101
variable_sites
38
pi
0.0242
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCGGTCTTACAAACTC CGGGGGTTTTTACGGAACTT 50.0 50.0 57.9 58.4 1081-1272 bp 1.0 82.9
trnK-UUU-rps16_p2 GTCGCGGTCTTACAAACTCT CGGGGGTTTTTACGGAACTT 50.0 50.0 57.9 58.4 1080-1271 bp 1.0 82.9
trnK-UUU-rps16_p3 AGTCGCGGTCTTACAAACTC TTGAAAAAGGGGCTCAACCT 50.0 45.0 57.9 57.5 1132-1323 bp 1.0 82.2
trnK-UUU-rps16_p4 GTCGCGGTCTTACAAACTCT TTGAAAAAGGGGCTCAACCT 50.0 45.0 57.9 57.5 1131-1322 bp 1.0 82.2
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CGGGGGTTTTTACGGAACTT 50.0 50.0 58.2 58.4 997-1188 bp 1.0 82.2