Hotspot

ycf1

SSC · 123575-129055 · 5481 bp

Back to Primulina

Pi 0.0165

MarkerSeek score 62.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5481
variable_sites: 481
pi: 0.0165
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGACCATTCAGGAAGTTGCA	TGGGTAGGAAAAAGGAAAGT	45.0	40.0	57.0	54.0	261-264 bp	1.0	49.5
ycf1_p2	AGACCATTCAGGAAGTTGCA	TTGGGTAGGAAAAAGGAAAGT	45.0	38.1	57.0	54.9	262-265 bp	1.0	48.9
ycf1_p3	TCCTCAGACCATTCAGGAAGT	TGGGTAGGAAAAAGGAAAGT	47.6	40.0	58.1	54.0	266-269 bp	1.0	48.9
ycf1_p4	TCCTCAGACCATTCAGGAAGT	TTGGGTAGGAAAAAGGAAAGT	47.6	38.1	58.1	54.9	267-270 bp	1.0	48.9
ycf1_p5	TCAGACCATTCAGGAAGTTGC	TGGGTAGGAAAAAGGAAAGT	47.6	40.0	58.2	54.0	263-266 bp	1.0	48.9