Hotspot

rps15-ycf1

SSC · 123199-123574 · 376 bp

Back to Primulina

Pi 0.0236

MarkerSeek score 63.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 376
variable_sites: 47
pi: 0.0236
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	AGACCCCATTCATTTGCCTG	45.0	50.0	58.1	58.1	1165-1380 bp	1.0	90.2
rps15-ycf1_p2	TCCCAACGCGTTACATAAGG	AGACCCCATTCATTTGCCTG	50.0	50.0	58.0	58.1	1302-1517 bp	1.0	90.0
rps15-ycf1_p3	TCCCAACGCGTTACATAAGG	ATTTGCCTGGAAACGACGAT	50.0	45.0	58.0	58.2	1291-1506 bp	1.0	89.8
rps15-ycf1_p4	CTCGAATACCCAACTCACCG	AGACCCCATTCATTTGCCTG	55.0	50.0	58.1	58.1	974-1190 bp	0.818182	81.2
rps15-ycf1_p5	TCGAGTCATTCTTCCATGCG	AGACCCCATTCATTTGCCTG	50.0	50.0	58.1	58.1	1253-1294 bp	0.409091	57.7