Hotspot

ndhF-rpl32

SSC · 112412-113157 · 746 bp

Back to Primulina

Pi 0.0282

MarkerSeek score 65.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 746
variable_sites: 84
pi: 0.0282
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.59
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGACCACATACGACGAAGA	ACGATGCACAATACCCCTTC	50.0	50.0	58.2	58.0	621-955 bp	0.590909	71.8
ndhF-rpl32_p2	AATAGAGCAGCACACAACGT	ACGATGCACAATACCCCTTC	45.0	50.0	57.8	58.0	1195-1529 bp	0.590909	70.1
ndhF-rpl32_p3	AGCAGCACACAACGTAAGAA	ACGATGCACAATACCCCTTC	45.0	50.0	58.1	58.0	1204-1524 bp	0.136364	49.3
ndhF-rpl32_p4	AGCAGCACACAACGTAAGAA	AATTTTTCGAGTCAGCCCGA	45.0	45.0	58.1	57.8	1328-1648 bp	0.136364	48.2
ndhF-rpl32_p5	AGCAGCACACAACGTAAGAA	TTTTTCGAGTCAGCCCGATT	45.0	45.0	58.1	57.8	1326-1646 bp	0.136364	48.2